Review



pgreenlantern gfp dna  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher pgreenlantern gfp dna
    Pgreenlantern Gfp Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 465742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgreenlantern gfp dna/product/Thermo Fisher
    Average 99 stars, based on 465742 article reviews
    pgreenlantern gfp dna - by Bioz Stars, 2026-04
    99/100 stars

    Images



    Similar Products

    pgreenlantern gfp dna  (Thermo Fisher)
    99
    Thermo Fisher pgreenlantern gfp dna
    Pgreenlantern Gfp Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgreenlantern gfp dna/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    pgreenlantern gfp dna - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    pgreenlantern-1 (5,030 bp) coding for gfp under the control of cmv promoter  (Thermo Fisher)
    90
    Thermo Fisher pgreenlantern-1 (5,030 bp) coding for gfp under the control of cmv promoter
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    Pgreenlantern 1 (5,030 Bp) Coding For Gfp Under The Control Of Cmv Promoter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgreenlantern-1 (5,030 bp) coding for gfp under the control of cmv promoter/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    pgreenlantern-1 (5,030 bp) coding for gfp under the control of cmv promoter - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    pgreenlantern (gfp)  (Amaxa)
    90
    Amaxa pgreenlantern (gfp)
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    Pgreenlantern (Gfp), supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgreenlantern (gfp)/product/Amaxa
    Average 90 stars, based on 1 article reviews
    pgreenlantern (gfp) - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    gfp cdna (pgreenlantern  (Thermo Fisher)
    90
    Thermo Fisher gfp cdna (pgreenlantern
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    Gfp Cdna (Pgreenlantern, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp cdna (pgreenlantern/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    gfp cdna (pgreenlantern - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    gfp plasmid pgreenlantern  (Promega)
    90
    Promega gfp plasmid pgreenlantern
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    Gfp Plasmid Pgreenlantern, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp plasmid pgreenlantern/product/Promega
    Average 90 stars, based on 1 article reviews
    gfp plasmid pgreenlantern - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    pgreenlantern gfp  (Thermo Fisher)
    86
    Thermo Fisher pgreenlantern gfp
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    Pgreenlantern Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgreenlantern gfp/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    pgreenlantern gfp - by Bioz Stars, 2026-04
    86/100 stars
    		  Buy from Supplier
    pgreenlantern plasmid encoding gfp  (Thermo Fisher)
    90
    Thermo Fisher pgreenlantern plasmid encoding gfp
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    Pgreenlantern Plasmid Encoding Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgreenlantern plasmid encoding gfp/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    pgreenlantern plasmid encoding gfp - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    pgreenlantern gfp in pcdna3  (Thermo Fisher)
    86
    Thermo Fisher pgreenlantern gfp in pcdna3
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    Pgreenlantern Gfp In Pcdna3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgreenlantern gfp in pcdna3/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    pgreenlantern gfp in pcdna3 - by Bioz Stars, 2026-04
    86/100 stars
    		  Buy from Supplier
    s65t gfp gene from pgreenlantern  (Thermo Fisher)
    90
    Thermo Fisher s65t gfp gene from pgreenlantern
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    S65t Gfp Gene From Pgreenlantern, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s65t gfp gene from pgreenlantern/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    s65t gfp gene from pgreenlantern - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    the modi®ed gfp-coding sequence was from vector pgreenlantern  (Thermo Fisher)
    90
    Thermo Fisher the modi®ed gfp-coding sequence was from vector pgreenlantern
    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of <t>pGreenLantern-1</t> (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
    The Modi®Ed Gfp Coding Sequence Was From Vector Pgreenlantern, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/the modi®ed gfp-coding sequence was from vector pgreenlantern/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    the modi®ed gfp-coding sequence was from vector pgreenlantern - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Immunohistochemistry, Transfection

    A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. B. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. B. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Immunohistochemistry, Transfection

    A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Double Immunofluorescence Staining, Transfection, Immunofluorescence, Software

    A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM (n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM (n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Double Immunofluorescence Staining, Transfection, Immunofluorescence, Software

    Representative micrographs of double immunofluorescence to TH and β-III-tubulin. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. The scale bar is common for all micrographs.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: Representative micrographs of double immunofluorescence to TH and β-III-tubulin. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. The scale bar is common for all micrographs.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Immunofluorescence, Transfection

    A . Sholl diagram for the morphological study of dendrites and dendritic spines. B . Representative micrograph of a medium spiny neuron (MSN) of a healthy striatum. The arrow shows the 50 μm segment (primary dendrite) where dendritic spines were analyzed. Upper calibration bar = 25 μm. The small panels show different types of spines (thin, mushroom, stubby and wide, from left to right) indicated by the arrow. Calibration bar = 5 μm. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion and the Sholl analysis was performed at the end of the study (12 weeks after transfection or 24 weeks after lesion). UT = untransfected rats with lesion. C. Sholl analysis of total number of intersections along dendritic trees in MSNs at all distances in 200-μm radius from the soma. D. Plot of mean spine density analyzed per 50-μm primary dendrite from cell body (proximal segment, upper arrow in B ) from six neurons per rat. All values represent the mean ± SEM ( n = 6 independent rats in each experimental condition). Statistical analysis ( C and D ) was performed using one-way ANOVA and Tukey post-hoc test.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: A . Sholl diagram for the morphological study of dendrites and dendritic spines. B . Representative micrograph of a medium spiny neuron (MSN) of a healthy striatum. The arrow shows the 50 μm segment (primary dendrite) where dendritic spines were analyzed. Upper calibration bar = 25 μm. The small panels show different types of spines (thin, mushroom, stubby and wide, from left to right) indicated by the arrow. Calibration bar = 5 μm. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion and the Sholl analysis was performed at the end of the study (12 weeks after transfection or 24 weeks after lesion). UT = untransfected rats with lesion. C. Sholl analysis of total number of intersections along dendritic trees in MSNs at all distances in 200-μm radius from the soma. D. Plot of mean spine density analyzed per 50-μm primary dendrite from cell body (proximal segment, upper arrow in B ) from six neurons per rat. All values represent the mean ± SEM ( n = 6 independent rats in each experimental condition). Statistical analysis ( C and D ) was performed using one-way ANOVA and Tukey post-hoc test.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Transfection

    Ipsilateral circling behavior ( A and C ) was activated by methamphetamine (8 mg/kg; i.p.), and contralateral circling behavior ( B and D ) was activated by apomorphine (0.5 mg/kg; i.p.) at weeks 0, 4 and 12 after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion (Week 0). All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P <0.0005, ** P < 0.0001.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: Ipsilateral circling behavior ( A and C ) was activated by methamphetamine (8 mg/kg; i.p.), and contralateral circling behavior ( B and D ) was activated by apomorphine (0.5 mg/kg; i.p.) at weeks 0, 4 and 12 after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion (Week 0). All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P <0.0005, ** P < 0.0001.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Transfection

    The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. UT = untransfected rats. The tests were performed at week 4 (12WL4WT) and week 12 (12WL12WT) after transfection. All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). * P < 0.001 when compared the values of the contralateral limb (Contra limb) with those of the ipsilateral limb (Ipsi limb), ** P < 0.001 when compared NRTN-His vs UT and § P < 0.001, NRTN-His vs GFP using two-way ANOVA. The post-hoc test was Newman-Keuls analysis.

    Journal: PLoS ONE

    Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion

    doi: 10.1371/journal.pone.0188239

    Figure Lengend Snippet: The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. UT = untransfected rats. The tests were performed at week 4 (12WL4WT) and week 12 (12WL12WT) after transfection. All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). * P < 0.001 when compared the values of the contralateral limb (Contra limb) with those of the ipsilateral limb (Ipsi limb), ** P < 0.001 when compared NRTN-His vs UT and § P < 0.001, NRTN-His vs GFP using two-way ANOVA. The post-hoc test was Newman-Keuls analysis.

    Article Snippet: Full NRTN sequence and correct Tag addition were confirmed by automatic sequencing (BigDye v3.1; Applied Biosystems; Foster City, CA, USA). pGreenLantern-1 (5,030 bp) coding for GFP under the control of CMV promoter (Gibco BRL; Grand Island, NY, USA) was used as a negative control.

    Techniques: Transfection